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An Improved, Rapid, and Sensitive Ultra-High-Performance Liquid Chromatography-High-Resolution Orbitrap Mass Spectrometry Analysis for the Determination of Highly Polar Pesticides and Contaminants in Processed Fruits and Vegetables
- Savini, Sara, Bandini, Mirella, Sannino, Anna
- Journal of agricultural and food chemistry 2019 v.67 no.9 pp. 2716-2722
- derivatization, food analysis, fruits, glyphosate, high performance liquid chromatography, infant foods, ion exchange chromatography, isotope labeling, mass spectrometry, perchlorates, phosphorous acid, polar compounds, ultra-performance liquid chromatography, vegetables
- A rapid, specific, and sensitive method based on quick polar pesticide extraction and ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry with an Orbitrap analyzer was evaluated. Usually, pesticides were analyzed individually using derivatization or ion-pairing techniques and detection by ion chromatography. We identified and simultaneously quantified 6 highly polar compounds (glyphosate, aminomethyl phosphonic acid (AMPA), phosphonic acid, fosetyl-Al, chlorate, and perchlorate) in 83 processed fruits and vegetables as well as 15 infant foods. Isotopically labeled internal standards ¹⁸O₄-perchlorate, ¹⁸O₃-chlorate, and ¹³C₂¹⁵N-glyphosate were applied to quantify five polar compounds and to compensate for any factor affecting the recovery rates. Only AMPA was quantified using a standard addition approach to compensate for matrix effects. This analytical methodology is fast and reliable, and it is also able to satisfy the strict requirements of infant food analysis.