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An Improved, Rapid, and Sensitive Ultra-High-Performance Liquid Chromatography-High-Resolution Orbitrap Mass Spectrometry Analysis for the Determination of Highly Polar Pesticides and Contaminants in Processed Fruits and Vegetables

Savini, Sara, Bandini, Mirella, Sannino, Anna
Journal of agricultural and food chemistry 2019 v.67 no.9 pp. 2716-2722
derivatization, food analysis, fruits, glyphosate, high performance liquid chromatography, infant foods, ion exchange chromatography, isotope labeling, mass spectrometry, perchlorates, phosphorous acid, polar compounds, ultra-performance liquid chromatography, vegetables
A rapid, specific, and sensitive method based on quick polar pesticide extraction and ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry with an Orbitrap analyzer was evaluated. Usually, pesticides were analyzed individually using derivatization or ion-pairing techniques and detection by ion chromatography. We identified and simultaneously quantified 6 highly polar compounds (glyphosate, aminomethyl phosphonic acid (AMPA), phosphonic acid, fosetyl-Al, chlorate, and perchlorate) in 83 processed fruits and vegetables as well as 15 infant foods. Isotopically labeled internal standards ¹⁸O₄-perchlorate, ¹⁸O₃-chlorate, and ¹³C₂¹⁵N-glyphosate were applied to quantify five polar compounds and to compensate for any factor affecting the recovery rates. Only AMPA was quantified using a standard addition approach to compensate for matrix effects. This analytical methodology is fast and reliable, and it is also able to satisfy the strict requirements of infant food analysis.