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Biosensing with Insect Odorant Receptor Nanodiscs and Carbon Nanotube Field-Effect Transistors

Author:
Murugathas, Thanihaichelvan, Zheng, Han Yue, Colbert, Damon, Kralicek, Andrew V., Carraher, Colm, Plank, Natalie O. V.
Source:
ACS applied materials & interfaces 2019 v.11 no.9 pp. 9530-9538
ISSN:
1944-8252
Subject:
Drosophila melanogaster, biosensors, carbon nanotubes, detection limit, electronic nose, insects, ligands, lipids, methyl salicylate, odor compounds, odorant receptors, transistors
Abstract:
Insect odorant receptors have been reconstituted into lipid nanodiscs and tethered to carbon nanotube field-effect transistors to function as a biosensor. Here, four different insect odorant receptors (ORs) from Drosophila melanogaster (DmelOR10a, DmelOR22a, DmelOR35a, and DmelOR71a) were expressed in Sf9 cells, purified, and reconstituted into lipid nanodiscs. We have demonstrated that each of these ORs produce a selective and highly sensitive electrical response to their respective positive ligands, methyl salicylate, methyl hexanoate, trans-2-hexen-1-al, and 4-ethylguaiacol, with limits of detection in the low femtomolar range. No detection was observed for each OR against control ligands, and empty nanodiscs showed no specific sensor signal for any of the odorant molecules. Our results are the first evidence that insect ORs can be integrated into lipid nanodiscs and used as primary sensing elements for bioelectronic nose technologies.
Agid:
6323608