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Nanocellulose biosynthesis by Komagataeibacter hansenii in a defined minimal culture medium

de Souza, Samara S., Berti, Fernanda V., de Oliveira, Karla P. V., Pittella, Camila Q. P., de Castro, Julia V., Pelissari, Catiane, Rambo, Carlos R., Porto, Luismar M.
Cellulose 2019 v.26 no.3 pp. 1641-1655
ammonium chloride, ammonium nitrate, ammonium sulfate, biosynthesis, carbon, cellulose, culture media, fructose, glucose, glycerol, mannitol, nitrogen, nutrients, peptones, yeast extract
This study evaluates the capability of Komagataeibacter hansenii ATCC 23769 to synthesize bacterial nanocellulose (BNC) under strict limitation of nutrients with different carbon and nitrogen sources in a defined minimal culture medium in static culture. Five carbon sources were prepared based on the C-molar basis concentration: glycerol, glucose, fructose, mannitol and saccharose, combined with three different nitrogen sources: NH₄Cl, NH₄NO₃ and (NH₄)₂SO₄. BNC production yields were determined based on the dry weight of the produced BNC-Minimal membranes and the carbon consumption for each condition. The combination of 25 mM of glucose and 10 mM of NH₄Cl showed the best concentration of C and N sources regarding BNC yield and membrane stability. This is a new proposal of defined minimal culture medium that supports growth of BNC membranes production, without the addition of complex elements such as yeast extract and peptone. BNC membranes produced under these conditions exhibited great optical transparency compared to the membranes produced in complex media (Mannitol and Hestrin–Schramm), therefore increasing a wide range of technological applications.