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Development of quantitative PCR primers and probes for environmental DNA detection of amphibians in Ontario
- Beauclerc, Kaela, Wozney, Kristyne, Smith, Caleigh, Wilson, Chris
- Conservation genetics resources 2019 v.11 no.1 pp. 43-46
- DNA, DNA primers, amphibians, indigenous species, monitoring, quantitative polymerase chain reaction, Ontario
- DNA from environmental samples (eDNA) is increasingly being used to detect and monitor elusive species. We developed species-specific eDNA primers and probes for qPCR detection of 24 amphibian species native to Ontario, Canada. Cross-species testing confirmed their high specificity and low cross-species amplification, as well as their ability to detect DNA from target species at low concentrations. These detection tools should prove useful for monitoring at-risk amphibian species throughout their native ranges.