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Stingless bee honey protects against lipopolysaccharide induced-chronic subclinical systemic inflammation and oxidative stress by modulating Nrf2, NF-κB and p38 MAPK

Ranneh, Yazan, Akim, Abdah Md, Hamid, Hasiah Ab, Khazaai, Huzwah, Fadel, Abdulmannan, Mahmoud, Ayman M.
Nutrition & metabolism 2019 v.16 no.1 pp. 15
antioxidants, arthritis, blood serum, chronic diseases, heart, histology, inflammation, interleukin-1beta, interleukin-6, interleukin-8, intraperitoneal injection, kidneys, laboratory animals, lipopolysaccharides, liver, lungs, males, malondialdehyde, mitogen-activated protein kinase, neoplasms, neurodegenerative diseases, noninsulin-dependent diabetes mellitus, oxidative stress, protective effect, rats, risk factors, stingless bees, transcription factor NF-kappa B, tumor necrosis factor-alpha
BACKGROUND: Epidemiological and experimental studies have extensively indicated that chronic subclinical systemic inflammation (CSSI) and oxidative stress are risk factors for several chronic diseases, including cancer, arthritis, type 2 diabetes, and cardiovascular and neurodegenerative diseases. This study examined the protective effect of stingless bee honey (SBH) supplementation against lipopolysaccharide (LPS)-induced CSSI, pointing to the possible involvement of NF-κB, p38 MAPK and Nrf2 signaling. METHODS: CSSI was induced in male Sprague Dawley rats by intraperitoneal injection of LPS three times per week for 28 days, and SBH (4.6 and 9.3 g/kg/day) was supplemented for 30 days. RESULTS: LPS-induced rats showed significant leukocytosis, and elevated serum levels of CRP, TNF-α, IL-1β, IL-6, IL-8, MCP-1, malondialdehyde (MDA) and 8-hydroxy-2′-deoxyguanosine (8-OHdG), accompanied with diminished antioxidants. Treatment with SBH significantly ameliorated inflammatory markers, MDA and 8-OHdG, and enhanced antioxidants in LPS-induced rats. In addition, SBH decreased NF-κB p65 and p38 MAPK, and increased Nrf2 expression in the liver, kidney, heart and lung of LPS-induced rats. Furthermore, SBH prevented LPS-induced histological and functional alterations in the liver, kidney, heart and lung of rats. CONCLUSION: SBH has a substantial protective role against LPS-induced CSSI in rats mediated via amelioration of inflammation, oxidative stress and NF-κB, p38 MAPK and Nrf2 signaling.