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14-3-3ε acts as a proviral factor in highly pathogenic porcine reproductive and respiratory syndrome virus infection

Author:
Cao, Shengliang, Cong, Fangyuan, Tan, Min, Ding, Guofei, Liu, Jiaqi, Li, Li, Zhao, Yuzhong, Liu, Sidang, Xiao, Yihong
Source:
Veterinary research 2019 v.50 no.1 pp. 16
ISSN:
1297-9716
Subject:
Porcine reproductive and respiratory syndrome virus, macrophages, porcine reproductive and respiratory syndrome, small interfering RNA, swine, therapeutics, vaccines, viral nonstructural proteins, China
Abstract:
The highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) emerged in 2006 in China and caused great economic losses for the swine industry because of the lack of an effective vaccine. 14-3-3 proteins are generating significant interest as potential drug targets by allowing the targeting of specific pathways to elicit therapeutic effects in human diseases. In a previous study, 14-3-3s were identified to interact with non-structural protein 2 (NSP2) of PRRSV. In the present study, the specific subtype 14-3-3ε was confirmed to interact with NSP2 and play a role in the replication of the HP-PRRSV TA-12 strain. Knockdown of 14-3-3ε in Marc-145 cells and porcine alveolar macrophages (PAMs) caused a significant decrease in TA-12 replication, while stable overexpression of 14-3-3ε caused a significant increase in the replication of TA-12 and low pathogenic PRRSV (LP-PRRSV) CH-1R. The 14-3-3 inhibitor difopein also decreased TA-12 and CH-1R replication in Marc-145 cells and PAMs. These findings are consistent with 14-3-3ε acting as a proviral factor and suggest that 14-3-3ε siRNA and difopein are therapeutic candidates against PRRSV infection.
Agid:
6325969