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A balanced game: chicken macrophage response to ALV-J infection

Author:
Feng, Min, Xie, Tingting, Li, Yuanfang, Zhang, Nan, Lu, Qiuyuan, Zhou, Yaohong, Shi, Meiqing, Sun, Jingchen, Zhang, Xiquan
Source:
Veterinary research 2019 v.50 no.1 pp. 20
ISSN:
1297-9716
Subject:
Avian leukosis virus, Western blotting, carrier state, chemokine CCL20, chickens, gene expression regulation, gene ontology, genes, immunosuppression, inducible nitric oxide synthase, innate immunity, interleukin-1beta, lysozyme, macrophages, neoplasms, pathogens, reverse transcriptase polymerase chain reaction, transcriptomics
Abstract:
Avian leukosis virus subgroup J (ALV-J) infection can cause tumors and immunosuppression in infected chickens. Macrophages play a central role in host defense against invading pathogens. In this study, we discovered an interesting phenomenon: ALV-J replication is weakened from 3 hours post-infection (hpi) to 36 hpi, which was verified using Western blotting and RT-PCR. To further investigate the interaction between ALV-J and macrophages, transcriptome analysis was performed to analyze the host genes’ function in chicken primary monocyte-derived macrophages (MDM). Compared to the uninfected control, 624 up-regulated differentially expressed genes (DEG) and 341 down-regulated DEG at 3 hpi, and 174 up-regulated DEG and 87 down-regulated DEG at 36 hpi were identified in chicken MDM, respectively. ALV-J infection induced strong innate immune responses in chicken MDM at 3 hpi, instead of 36 hpi, according to the analysis results of Gene Ontology and KEGG pathway. Importantly, the host factors, such as up-regulated MIP-3α, IL-1β, iNOS, K60, IRG1, CH25H, NFKBIZ, lysozyme and OASL were involved in the host defense response during the course of ALV-J infection. On the contrary, up-regulated EX-FABP, IL4I1, COX-2, NFKBIA, TNFAIP3 and the Jak STAT pathway inhibitors including CISH, SOCS1 and SOCS3 are beneficial to ALV-J survival in chicken macrophages. We speculated that ALV-J tropism for macrophages helps to establish a latent infection in chicken MDM from 6 to 36 hpi. The present study provides a comprehensive view of the interactions between macrophages and ALV-J. It suggests the mechanisms of defense of chicken macrophages against ALV-J invasion and how ALV-J escape the host innate immune responses.
Agid:
6325973