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In Vitro Antimicrobial Activity of Endophytes, Isolated from Moringa peregrina Growing in Eastern Region of Saudi Arabia

Author:
Aljuraifani, Amal, Aldosary, Sahar, Ababutain, Ibtisam
Source:
National Academy science letters 2019 v.42 no.1 pp. 75-80
ISSN:
0250-541X
Subject:
Acinetobacter, Bacillus licheniformis, Bacillus pumilus, Bacillus subtilis, Candida albicans, Enterobacter aerogenes, Moringa peregrina, Proteus mirabilis, Streptococcus pyogenes, animal pathogens, antimicrobial properties, bacteria, endophytes, growth retardation, minimum inhibitory concentration, nucleotide sequences, quantitative analysis, ribosomal RNA, Saudi Arabia
Abstract:
Seven endophytes bacteria were isolated from Moringa peregrina and identified using 16S ribosomal RNA sequences, which revealed two bacterial genuses, namely Proteus mirabilis and Bacillus; three species belong to Bacillus: B. licheniformis, B. subtilis subsp. Inaquosorum and B. pumilus. The antimicrobial activity of the isolate was screened against 11 human pathogens, and the potency of the selected isolate was qualitatively and quantitatively analyzed by the cross-streak method and minimum inhibitory concentrations (MICs), respectively. Minimum inhibitory concentration (MIC) of the organic extract of selected isolate B. licheniformis MpKL1 was found to exhibit appreciable growth inhibition between 125 µg/ml and 500 µg/ml against most tested human pathogens and less influence against Acinetobacter baumanii, Enterobacter aerogenes, Streptococcus pyogenes and Candida albicans with MIC > 1000.
Agid:
6326056