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Ameliorative effects of gallic acid on gentamicin-induced nephrotoxicity in rats

Ghaznavi, Habib, Fatemi, Iman, Kalantari, Heibatullah, Hosseini Tabatabaei, Seyed Mohammad Taghi, Mehrabani, Mehrnaz, Gholamine, Babak, Kalantar, Mojtaba, Mehrzadi, Saeed, Goudarzi, Mehdi
Journal of Asian natural products research 2018 v.20 no.12 pp. 1182-1193
adverse effects, blood sampling, blood serum, catalase, creatinine, drugs, gallic acid, gentamicin, glutathione, glutathione peroxidase, histology, kidneys, laboratory animals, males, malondialdehyde, nephroprotective effect, nephrotoxicity, nitric oxide, rats, superoxide dismutase, urea nitrogen
The major side effect of gentamicin (GEN) is nephrotoxicity which in turn restricts the clinical use of this drug. In this study, the effect of gallic acid (GA) on gentamicin-induced nephrotoxicity was studied. A total number of 28 male Wistar rats were randomly divided into four experimental groups: control, GEN (100 mg/kg/day), GEN + GA (30 mg/kg/day), GA (30 mg/kg/day). All drug administrations were done intraperitoneally (i.p) for eight consecutive days. Twenty-four hours after the last administration, blood samples were collected to determine serum creatinine (Cr), blood urea nitrogen (BUN). The right kidney was used for histological examination. Malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO) levels and catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activity were assayed in left renal tissue. Results showed a significant increase in the levels of MDA, NO, Cr, and BUN and decrease of GSH, CAT, GPx, and SOD by GEN administration. Co-administration with GA showed reduction in the levels of MDA, NO, Cr, and BUN and increase in GSH, CAT, GPx, and SOD. Also, the nephroprotective effect of GA was confirmed by the histological examination of the kidneys. The results of our study showed that GA exerts a significant nephroprotective effect against GEN-induced nephrotoxicity.