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Confirmation of Fusarium root rot resistance QTL Fsp-Ps 2.1 of pea under controlled conditions

Coyne, Clarice J., Porter, Lyndon D., Boutet, Gilles, Ma, Yu, McGee, Rebecca J., Lesné, Angélique, Baranger, Alain, Pilet-Nayel, Marie-Laure
BMC plant biology 2019 v.19 no.1 pp. 98
Fusarium solani f. sp. pisi, alleles, breeding programs, chromosome mapping, confidence interval, disease severity, fungi, genetic resistance, genotyping by sequencing, greenhouse experimentation, marker-assisted selection, peas, plant genetic resources, quantitative trait loci, root rot, single nucleotide polymorphism, variance, North America
BACKGROUND: Dry pea production has increased substantially in North America over the last few decades. With this expansion, significant yield losses have been attributed to an escalation in Fusarium root rots in pea fields. Among the most significant rot rotting pathogenic fungal species, Fusarium solani fsp. pisi (Fsp) is one of the main causal agents of root rot of pea. High levels of partial resistance to Fsp has been identified in plant genetic resources. Genetic resistance offers one of the best solutions to control this root rotting fungus. A recombinant inbred population segregating for high levels of partial resistance, previously single nucleotide polymorphism (SNP) genotyped using genotyping-by-sequencing, was phenotyped for disease reaction in replicated and repeated greenhouse trials. Composite interval mapping was deployed to identify resistance-associated quantitative trait loci (QTL). RESULTS: Three QTL were identified using three disease reaction criteria: root disease severity, ratios of diseased vs. healthy shoot heights and dry plant weights under controlled conditions using pure cultures of Fusarium solani fsp. pisi. One QTL Fsp-Ps 2.1 explains 44.4–53.4% of the variance with a narrow confidence interval of 1.2 cM. The second and third QTL Fsp-Ps3.2 and Fsp-Ps3.3 are closely linked and explain only 3.6–4.6% of the variance. All of the alleles are contributed by the resistant parent PI 180693. CONCLUSION: With the confirmation of Fsp-Ps 2.1 now in two RIL populations, SNPs associated with this region make a good target for marker-assisted selection in pea breeding programs to obtain high levels of partial resistance to Fusarium root rot caused by Fusarium solani fsp. pisi.