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Rapid and non-desctructive detection of Wolbachia in Aedes aegypti by near-infrared spectroscopy
- Maggy T. Sikulu-Lord, Marta F. Maia, Masabho P. Milali, Michael Henry, Gustav Mkandawile, Elise A. Kho, Robert A. Wirtz, Leon E. Hugo, Floyd E. Dowell, Gregor J. Devine
- PLoS Neglected Tropical Diseases 2016 v.10 no.6 pp. e0004759
- Aedes aegypti, Wolbachia pipientis, biological control agents, diagnostic techniques, disease control, females, males, microbial detection, monitoring, near-infrared spectroscopy, nondestructive methods, polymerase chain reaction, rapid methods
- The release of Wolbachia infected mosquitoes is likely to form a key component of disease control strategies in the near future. We investigated the potential of using near-infrared spectroscopy (NIRS) to simultaneously detect and identify two strains of Wolbachia pipien- tis (wMelPop and wMel) in male and female laboratory-reared Aedes aegypti mosquitoes. Our aim is to find faster, cheaper alternatives for monitoring those releases than the molecu- lar diagnostic techniques that are currently in use. Our findings indicate that NIRS can differ- entiate females and males infected with wMelPop from uninfected wild type samples with an accuracy of 96% (N = 299) and 87.5% (N = 377), respectively. Similarly, females and males infected with wMel were differentiated from uninfected wild type samples with accura- cies of 92% (N = 352) and 89% (N = 444). NIRS could differentiate wMelPop and wMel transinfected females with an accuracy of 96.6% (N = 442) and males with an accuracy of 84.5% (N = 443). This non-destructive technique is faster than the standard polymerase chain reaction diagnostic techniques. After the purchase of a NIRS spectrometer, the technique requires little sample processing and does not consume any reagents.