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Proteome modifications of Pacific white shrimp (Litopenaeus vannamei) muscle under biofloc system

Abbaszadeh, Akbar, Keyvanshokooh, Saeed, Yavari, Vahid, Naderi, Mahdi
Aquaculture nutrition 2019 v.25 no.2 pp. 358-366
H+/K+-exchanging ATPase, H-transporting ATP synthase, Litopenaeus vannamei, arginine kinase, biofloc technology, crude protein, diet, enzyme inhibitors, fiberglass, lactate dehydrogenase, metabolism, mitochondria, muscle tissues, muscles, myosin heavy chains, phosphopyruvate hydratase, proteome, proteomics, shrimp, tanks
The objective of the study was to examine the effects of biofloc technology on the muscle proteome of Litopenaeus vannamei. Two biofloc treatments and one control were compared: biofloc‐based tanks under zero‐water exchange fed with 150 g/kg crude protein (BF15), or with 250 g/kg crude protein (BF25) diets, and clear water tanks with 50% of daily water exchange stocked with shrimp fed with similar amount of a 250 g/kg crude protein diet, referred to as control. The shrimp (5.28 ± 0.42 g) were divided into the 300‐L fibreglass tanks (water volume of 200 L) at a density of 35 shrimp per tank and were cultured for 35 days. The biofloc groups displayed better growth and survival compared to the control. The muscle tissue from the control and BF25 groups was subjected to proteomic analysis. Lactate dehydrogenase, enolase, arginine kinase, mitochondrial ATP synthase subunit alpha, mitochondrial ATPase inhibitor factor 1 precursor, serpin 3 and myeloid differentiation factor 88 had an increased abundance in the BF25 group, while myosin heavy chain type 1 and myosin heavy chain type 2 showed a decreased abundance. The results indicate that biofloc technology could alter the expression of proteins involved in structure, metabolism and immune status of cultured shrimp.