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In vitro selection and in vivo confirmation of the antioxidant ability of Lactobacillus brevis MG000874

Author:
Noureen, S., Riaz, A., Arshad, M., Arshad, N.
Source:
Journal of applied microbiology 2019 v.126 no.4 pp. 1221-1232
ISSN:
1364-5072
Subject:
Lactobacillus brevis, albino, animal models, antioxidant activity, blood serum, catalase, galactose, glutathione, glutathione transferase, humans, kidney function tests, kidneys, lactic acid bacteria, lipid peroxidation, liver, metabolites, mice, oxidative stress, probiotics, reactive oxygen species, safety assessment, sequence analysis, subcutaneous injection, superoxide dismutase, tissues
Abstract:
AIMS: This study aimed at isolating a probiotic strain from a collection of lactic acid bacteria (LAB) with the high antioxidant property and confirms its potential in d‐gal‐induced oxidative stress (OS) murine model. METHODS AND RESULTS: The in vitro antioxidant ability of 16 LAB strains was determined in the cell‐free supernatant of 3‐ to 5‐day‐old culture, intact cells and cell lysates using three different methods for determining the reactive oxygen species scavenging activity and inhibition of lipid peroxidation. The strain, An28, presented the best antioxidant activity and was identified as Lactobacillus brevis MG000874 on the basis of 16 sRNA gene sequencing. The antioxidant potential of L. brevis MG000874 was confirmed in an OS murine model. Albino mice were exposed to d‐galactose at a dose of 150 mg kg⁻¹ BW and fed with L. brevis MG000874 (0·2 ml of 10¹⁰CFU per ml cell suspension per animal per day). Antioxidant enzymes were quantified spectrophotometrically in the liver, kidney and serum. Subcutaneous administration of d‐gal resulted in decrease in superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) and increase in glutathione‐S‐transferase (GST) levels in animals. The L. brevis MG000874‐treated animals displayed improvement in SOD, CAT and GST in all tissues and GSH in the liver and serum. The safety assessment of L. brevis MG000874 was performed by comparing liver and renal function tests. None of the selected indicators was significantly different from the negative control group. CONCLUSIONS: The antioxidant potential of 16 strains was noticed to be strain specific and in vivo performance of L. brevis MG000874 was found satisfactory in a d‐gal murine model. SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobacillus brevis MG000874 was identified for its admirable antioxidant property. This strain or/and its metabolites could be further investigated for possible applications in humans and veterinary fields.
Agid:
6327204