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Complete nucleotide sequence of a novel mycovirus from Trichoderma harzianum in China

Author:
Liu, Chenchen, Li, Mei, Redda, Estifanos Tsegaye, Mei, Jie, Zhang, Jiantai, Elena, Santiago F., Wu, Beilei, Jiang, Xiliang
Source:
Archives of virology 2019 v.164 no.4 pp. 1213-1216
ISSN:
0304-8608
Subject:
RNA-directed RNA polymerase, Trichoderma harzianum, double-stranded RNA, metagenomics, mycoviruses, open reading frames, phylogeny, reverse transcriptase polymerase chain reaction, China
Abstract:
A new mycovirus was identified in Trichoderma harzianum strain 137 isolated in Xinjiang province, China. The whole genome sequence of the mycovirus was determined by metagenomic sequencing, RT-PCR, and RACE cloning. The mycovirus contained two genomic segments. The first segment was 2088 bp long and contained a single ORF (ORF1) encoding the RNA-dependent RNA polymerase (RdRP) (72.26 kDa). The second segment was 1634 bp long and also contained a single ORF (ORF2) encoding a hypothetical protein of 37.472 kDa. We named this novel mycovirus “Trichoderma harzianum bipartite mycovirus 1” (ThBMV1). Phylogenetic analysis showed that ThBMV1 clusters with other unclassified dsRNA mycoviruses.
Agid:
6327479