Main content area

Evaluation of mycotoxins and their estimated daily intake in popcorn and cornflakes using LC-MS techniques

Andrade, G.C.R.M., Pimpinato, R.F., Francisco, J.G., Monteiro, S.H., Calori-Domingues, M.A., Tornisielo, V.L.
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.95 pp. 240-246
T-2 toxin, aflatoxin B1, average daily intake, dietary exposure, electrospray ionization mass spectrometry, fumonisins, liquid chromatography, monitoring, neosolaniol, popcorn, tandem mass spectrometry
The occurrence of 11 mycotoxins in samples of popcorn and cornflakes was evaluated by liquid chromatography electrospray ionization tandem mass spectrometry (LC-MS/MS), using dynamic multiple reaction monitoring. This quick, easy, cheap, effective, rugged and safe (QuEChERS) method was modified and provided good analytical results (recoveries ranged from 79 to 119%, RSDs <21% and LOQs below the established maximum level). Popcorn samples were contaminated with fumonisins, aflatoxin B1, neosolaniol and T-2 toxin, with mean concentrations of 592, 10.2 and 10.2 μg kg−1, respectively, while cornflake samples were contaminated with only T-2 (mean 11.2 μg kg−1). The co-occurrence of fumonisin B1+ aflatoxin B1 was observed in 52% of popcorn samples and was not observed in cornflake samples. The estimated intake for the Brazilian population of total fumonisins and T-2 toxin for popcorn as the upper bound approach were 0.0051 and 0.00004 μg kg −1 bw day −1, respectively, and for the 99th percentile were 0.1943 and 0.0014 μg kg −1 bw day −1, respectively, suggesting a low risk of ingestion. However heavy consumers may be exposed to a potential risk due to fumonisins (upper bound of 4.6 μg kg−1 bw day−1).