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Application of chemometric methods in the evaluation of antioxidants activity from degreased chia seeds extracts
- Laczkowski, Mirian S., Gonçalves, Thays R., Gomes, Sandra T.M., Março, Paulo Henrique, Valderrama, Patrícia, Matsushita, Makoto
- Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.95 pp. 303-307
- 2,2-diphenyl-1-picrylhydrazyl, Salvia, antioxidants, chemometrics, cold, ethanol, experimental design, least squares, models, near-infrared spectroscopy, pressing, seeds
- Samples of chia seeds (Salvia hispánica L.) were degreased by cold pressing and the residue obtained named degreased chia seeds (DCS) had the antioxidants extract obtained. A 22 experimental design was employed in order to evaluate the factors ethanol concentration and extraction time. The optimized conditions were extraction time 15 min with ethanol 70% where the activity (RSA), evaluated by the DPPH (2,2-diphenyl-1-picryl-hydrazyl) method was 94.76%. For this extract, the total phenolics content was 359.64 mg EGA/100 g. A pseudo-univariate calibration model is proposed as an alternative methodology to determine the %RSA by using the relative concentration from multivariate curve resolution with alternating least squares (MCR-ALS) from near-infrared (NIR) spectra of the antioxidant extract from DCS. The results suggest the possibility of %RSA determination directly from NIR spectra from extracts from DCS by using the multivariate resolution method MCR-ALS.