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Effect of P-glycoprotein regulation on cadmium exhaustion from Crassostrea gigas

Yang, Chao, Liu, Qingkang, Li, Guangzhen, Meng, Xianghong, Liu, Bingjie
Marine pollution bulletin 2019 v.141 pp. 307-312
Crassostrea gigas, P-glycoproteins, antioxidant activity, cadmium, lipids, metallothionein, rifampicin, seawater, verapamil, water pollution, xenobiotics
P-glycoprotein (P-gp) is a molecular pump, responsible for extruding xenobiotics. The aim of the study was to demonstrate the role of P-glycoprotein (P-gp) in cadmium (Cd) exhaustion. The activity of P-gp was regulated in Crassostrea gigas, which was previously exposed to Cd by using rifampicin (inducer) and verapamil (inhibitor), respectively. Comparing with Crassostrea gigas depurated in natural seawater, Cd content increased significantly from 14.28 mg/kg dw to 17.49 mg/kg dw accompanied by a changed metallothionein level from 9.84 μg/g fw to 10.67 μg/g fw after 25 μg/L verapamil treatment, while Cd content after 25 μg/L rifampicin treatment reduced to 12.21 mg/kg dw. Moreover, after treatment with rifampicin and verapamil, beneficial metal elements, fats, and proteins were maintained, and the tissue-dependent difference was found in the variation of antioxidant defenses and oxidative damage in Crassostrea gigas. In brief, the study provided new evidence on possibility of Cd removal by inducing P-glycoprotein.