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Diversity and abundance of antibiotic resistance of bacteria during the seedling period in marine fish cage-culture areas of Hainan, China

Author:
Wu, Jinjun, Mao, Can, Deng, Yiqin, Guo, Zhixun, Liu, Guangfeng, Xu, Liwen, Bei, Lei, Su, Youlu, Feng, Juan
Source:
Marine pollution bulletin 2019 v.141 pp. 343-349
ISSN:
0025-326X
Subject:
Alteromonas, Vibrio, amoxicillin, antibiotic resistance, antibiotic resistance genes, bacteria, cage culture, erythromycin, furazolidone, gentamicin, marine fish, nitrofurantoin, quantitative polymerase chain reaction, secondary infection, seedlings, water pollution, China
Abstract:
Antibiotic resistance has become an important focus of research in the aquaculture environment. However, few studies have evaluated antibiotic resistance during the seedling period in marine fish cage-culture areas. In this study, culture-dependent methods and quantitative polymerase chain reaction were used to identify and detect cultivable heterotrophic antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs), respectively, during the seedling period in a marine fish cage-culture areas of Hainan, China. Bacterial resistance to amoxicillin, erythromycin, and gentamicin was generally high (average on 27.67%, 23.61% and 37.32%, respectively), whereas resistance to furazolidone and nitrofurantoin was generally low (average on 0.14% and 7.425%). Alteromonas (32.72%) and Vibrio (24.77%) were the dominant genus of ARB. Most ARB were opportunistic pathogens, belonging to the phylum Proteobacteria (96.02%). The abundance of sul family genes was higher than that of tet family genes. Overall, the abundance of ARGs and the resistance rates in HW was highest.
Agid:
6328388