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Diversity and abundance of antibiotic resistance of bacteria during the seedling period in marine fish cage-culture areas of Hainan, China

Wu, Jinjun, Mao, Can, Deng, Yiqin, Guo, Zhixun, Liu, Guangfeng, Xu, Liwen, Bei, Lei, Su, Youlu, Feng, Juan
Marine pollution bulletin 2019 v.141 pp. 343-349
Alteromonas, Vibrio, amoxicillin, antibiotic resistance, antibiotic resistance genes, bacteria, cage culture, erythromycin, furazolidone, gentamicin, marine fish, nitrofurantoin, quantitative polymerase chain reaction, secondary infection, seedlings, water pollution, China
Antibiotic resistance has become an important focus of research in the aquaculture environment. However, few studies have evaluated antibiotic resistance during the seedling period in marine fish cage-culture areas. In this study, culture-dependent methods and quantitative polymerase chain reaction were used to identify and detect cultivable heterotrophic antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs), respectively, during the seedling period in a marine fish cage-culture areas of Hainan, China. Bacterial resistance to amoxicillin, erythromycin, and gentamicin was generally high (average on 27.67%, 23.61% and 37.32%, respectively), whereas resistance to furazolidone and nitrofurantoin was generally low (average on 0.14% and 7.425%). Alteromonas (32.72%) and Vibrio (24.77%) were the dominant genus of ARB. Most ARB were opportunistic pathogens, belonging to the phylum Proteobacteria (96.02%). The abundance of sul family genes was higher than that of tet family genes. Overall, the abundance of ARGs and the resistance rates in HW was highest.