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Allelopathic impact of artificially applied coumarin on Fusarium oxysporum f.sp. niveum

Author:
Wu, Hong-Sheng, Raza, Waseem, Liu, Dong-Yang, Wu, Cheng-Long, Mao, Ze-Shen, Xu, Yang-Chun, Shen, Qi-Rong
Source:
World journal of microbiology & biotechnology 2008 v.24 no.8 pp. 1297-1304
ISSN:
0959-3993
Subject:
Fusarium oxysporum, Fusarium wilt, allelochemicals, biomass, conidia, coumarin, endo-1,4-beta-glucanase, enzyme activity, germination, growth retardation, hyphae, mycelium, mycotoxins, plant pathogens, proteinases, roots, signal transduction, sporulation, watermelons
Abstract:
Watermelon production is threatened by fusarium wilt caused by Fusarium oxysporum f.sp. niveum (FON) in continuous cultivation system. Some elements, mainly allelochemicals, released from living roots or decayed plants might be associated with the disease. The purpose of this work was to evaluate the possible impact of coumarin, one kind of watermelon allelochemical, on FON. Furthermore, possible new mechanisms might be investigated during the ecological interactions of plant-microbe. Results showed that coumarin strongly inhibited growth of FON leading to a decrease in its biomass, dry weight of mycelia of FON in a liquid culture. The dry weight was decreased by 62.9% compared with control. The hyphal growth of FON on plates was stopped at high (>400 mg l⁻¹) concentrations of coumarin. At 320 mg l⁻¹, sporulation and enzyme activities of FON were also severely suppressed by coumarin. The yield of conidia, and the activities of proteinase, cellulase, and amylase were reduced by 98.9%, 79.7%, 29.8% and 15.9% respectively. However, conidial germination and mycotoxin (MT) production of FON were greatly stimulated, being increased by 55.7% and 14.9 fold at 320 mg l⁻¹ respectively. We conclude that coumarin acted as an allelochemical substance to inhibit growth and pathogenic enzyme activities of FON but to stimulate mycotoxin production and conidial germination. It was suggested that coumarin acted as a signal transduction element bridging plant and pathogen in the process of plant-microbe interactions.
Agid:
632840