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Analysis of physiological responses and expression profiling of boron transporter-like genes in response to excess boron in Populus russkii

Author:
Ou, Yongbin, Wu, Xiuli, Gao, Yongfeng, Wu, Yingqing, Yao, Yinan
Source:
Chemosphere 2019 v.224 pp. 369-378
ISSN:
0045-6535
Subject:
Populus, antioxidant activity, boron, cytoplasm, genes, leaves, physiological response, phytoremediation, plant growth, polluted soils, proline, toxicity, yeasts
Abstract:
Poplars (Populus species) are tolerant to boron (B) toxicity and have phytoremediation potential in B-contaminated soils. However, the detoxification strategy is largely unknown. To screen the key B transporter-like (BOR-like) genes for B compartmentation, Populus russkii plants were exposed to different levels of excess B and the plant growth, physiological responses, B distribution, and the expression patterns of BOR-like genes were characterized. P. russkii showed moderate tolerance to excess B although the plant growth was inhibited. The enhanced proline level and well-regulated antioxidant defense system were associated with B tolerance in leaves. The B absorbed by plants was predominantly allocated to leaves. Ten BOR-like genes were identified and seven of them showed tissue-specific expression patterns. PrBOR7 was identified as an important BOR-like gene possibly involved in the export of B from leaf cytoplasm because it was expressed specifically in leaves and induced by excess B. Yeast experiment assays verified that PrBOR7 functions as an efflux-type transporter and strongly improved cell tolerance to excess B. The expression patterns of BOR-like genes highlight the diversity of the family members in P. russkii, and PrBOR7 has potential as a candidate gene for B detoxification.
Agid:
6328986