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Alleviation of heavy metal and silver nanoparticle toxicity and enhancement of their removal by hydrogen sulfide in Phanerochaete chrysosporium

Huang, Zhenzhen, He, Kai, Song, Zhongxian, Zeng, Guangming, Chen, Anwei, Yuan, Lei, Li, Hui, Chen, Guiqiu
Chemosphere 2019 v.224 pp. 554-561
Phanerochaete chrysosporium, abiotic stress, antioxidant activity, antioxidants, biotic stress, cadmium, catalase, cell communication, cell viability, copper, heavy metals, hydrogen sulfide, lead, lipid peroxidation, nanosilver, nickel, oxidative stress, silver, sodium, superoxide dismutase, toxic substances, toxicity, wastewater, zinc
Hydrogen sulfide (H2S), an important cellular signaling molecule, plays vital roles in mediating responses to biotic/abiotic stresses. Influences of H2S on metal removal, cell viability, and antioxidant response of Phanerochaete chrysosporium upon exposure to heavy metals and silver nanoparticles (AgNPs) in the present study were investigated. An enhancement in Pb(ΙΙ) removal with an increase in concentration of the H2S donor sodium hydrosulfide (NaHS) was observed, and the maximum removal efficiencies increased by 31% and 17% under 100 and 200 mg/L Pb(ΙΙ) exposure, respectively, in the presence of 500 μM NaHS. Application of 500 μM NaHS increased the cell viability by 15%–39% under Pb(II) stress (10–200 mg/L) with relative to the untreated control. Increase in total Ag uptake and cell survival was also elicited by NaHS in a concentration-dependent manner under AgNP stress. Meanwhile, activities of superoxide dismutase and catalase were significantly enhanced with the introduction of NaHS under stresses of Pb(II), Cd(II), Cu(II), Zn(II), Ni(II), and AgNPs. The inhibition in lipid peroxidation and oxidative stress was observed in P. chrysosporium cells exposed to these toxicants following NaHS pretreatment, which could be attributed to the upregulation in antioxidant enzymes. The results obtained suggest that H2S can alleviate heavy metals and AgNP-induced toxicity to P. chrysosporium and improve the removal efficiency of these toxicants from wastewater.