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Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions

Author:
Sarah M. Romereim, Adam F. Summers, William E. Pohlmeier, Pan Zhang, Xiaoying Hou, Heather A. Talbott, Robert A. Cushman, Jennifer R. Wood, John S. Davis, Andrea S. Cupp
Source:
Molecular and Cellular Endocrinology 2017 v.439 no. pp. 379-394
ISSN:
0303-7207
Subject:
RNA, cattle, enzymes, gene expression, gene expression regulation, genes, genetic markers, granulosa cells, luteal cells, microarray technology, ovulation, principal component analysis, quantitative polymerase chain reaction, receptors, somatic cells
Abstract:
After ovulation, somatic cells of the ovarian follicle (theca and granulosa cells) become the small and large luteal cells of the corpus luteum. Aside from known cell type-specific receptors and steroidogenic enzymes, little is known about the differences in the gene expression profiles of these four cell types. Analysis of the RNA present in each bovine cell type using Affymetrix microarrays yielded new cell-specific genetic markers, functional insight into the behavior of each cell type via Gene Ontology Annotations and Ingenuity Pathway Analysis, and evidence of small and large luteal cell lineages using Principle Component Analysis. Enriched expression of select genes for each cell type was validated by qPCR. This expression analysis offers insight into the lineage and differentiation process that transforms somatic follicular cells into luteal cells.
Agid:
63301
Datasets:
Data from: Transcriptomes of bovine ovarian follicular and luteal cells
Handle:
10113/63301