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Development of sugarcane resource for efficient fermentation of exopolysaccharide by using a novel strain of Kosakonia cowanii LT-1

Wang, Liying, Wu, Lingtian, Chen, Qiaoyu, Li, Sha, Zhu, Yibo, Wu, Jinnan, Chu, Jianlin, Wu, Shanshan
Bioresource technology 2019 v.280 pp. 247-254
ABC transporters, adenosine triphosphate, batch fermentation, biosynthesis, cost effectiveness, energy, exopolysaccharides, oxidative phosphorylation, raw materials, sucrose, sugarcane juice, transcriptomics, transferases
This work focuses on the development of non-food fermentation for the cost-effective biosynthesis of exopolysaccharide (EPS) by using a new strain of Kosakonia cowanii LT-1. This novel strain more efficiently utilizes sucrose for EPS production than other glycosyl donors. Comparative transcriptomic analysis is used to understand EPS synthesis promotion and the effects of sucrose on EPS biosynthesis. We speculate that ATP-binding cassette transporter, phosphotransferase, and two-component systems may be the most essential factors for EPS biosynthesis. The enhanced oxidative phosphorylation increases the synthesis rate of ATP to satisfy the energy demands for EPS production with sucrose as the substrate. Sugarcane juice, a cheap raw material, could improve the EPS yield in batch fermentation and achieve approximately 29.66% cost savings for substrate. Our work presents a promising non-food fermentation approach for the synthesis of high-value industrial products.