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Method to quantify cytokines and chemokines in mouse brain tissue using Bio-Plex multiplex immunoassays
- Manglani, Monica, Rua, Rejane, Hendricksen, Amy, Braunschweig, Daniel, Gao, Qian, Tan, Woei, Houser, Brett, McGavern, Dorian B., Oh, Kenneth
- Methods 2019 v.158 pp. 22-26
- blood serum, brain, chemical species, chemokines, immunoassays, mice
- This protocol describes how to prepare mouse brain tissue for quantification of multiple inflammatory mediators using a multiplex bead-based immunoassay. It is important to have methods that allow quantification of multiple analytes from small amounts of tissue. Bio-Plex is a Luminex xMAP-based multiplex bead-based immunoassay technology that permits simultaneous analysis of up to 100 analytes from a single tissue sample. This assay has been used extensively to investigate analytes in plasma and serum samples as well as cultured and primary cells. Here, we describe a method for simultaneous analysis of 33 different inflammatory cytokines and chemokines from mouse brain tissue using the Bio-Plex Pro Mouse Chemokine Panel 33-Plex.