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Lactoferrin stimulates the expression of vitamin D receptor in vitamin D deficient mice

Wang, Jingxuan, Li, Yixuan, Zhao, Liang, Ren, Fazheng, Guo, Huiyuan
Journal of functional foods 2019 v.55 pp. 48-56
Lachnospiraceae, Lactobacillus, Toll-like receptor 4, blood serum, cadherins, calcitriol receptors, colon, dysbiosis, glycoproteins, interleukin-6, intestinal microorganisms, lactoferrin, mice, milk, occludins, public health, transcription factor NF-kappa B, transforming growth factor beta, tumor necrosis factor-alpha, vitamin D deficiency
Vitamin D deficiency, a growing public health problem, is associated with elevated serum pro-inflammatory cytokines and gut microbial dysbiosis. Lactoferrin (LF), a strongly cationic glycoprotein, is an multifunctional components of milk. We explored the effects of oral administratation with LF (100 mg/kg and 1000 mg/kg) on intestine of vitamin D deficient mice. Our results showed that LF reduced the abundance of Oscillibacter, increased the proportion of Lachnospiraceae, Faecalibaculum and Lactobacillus. In addition, LF alleviated the elevated serum LBP, TNF-α, IL-6 and TGF-β induced by vitamin D deficiency. Notably, we found the expression of vitamin D receptor significantly increased after LF supplement in colon of vitamin D deficient mice. At the same time, LF reduced the expression of TLR-4, inhibited the activity of NF-κB P65 and enhanced the expression of intestinal barrier proteins, including ZO-1, occludin, claudin-1 as well as E-cadherin in the colon of vitamin D deficient mice.