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Diversity of epiphytic fungi on the surface of Kyoho grape berries during ripening process in summer and winter at Nanning region, Guangxi, China

Ding, Su, Li, Nan, Cao, Muming, Huang, Qiufeng, Chen, Guopin, Xie, Shuyu, Zhang, Jing, Cheng, Guo, Li, Wei
Fungal biology 2018
Acremonium, Alternaria, Aspergillus, Cladosporium, Colletotrichum, Fusarium, Penicillium, Pseudozyma, Rhodosporidium, Sporobolomyces, dominant species, epiphytes, farming systems, fruits, fungal communities, fungi, high-throughput nucleotide sequencing, ripening, subtropics, summer, t-test, table grapes, winter, China
The two-harvest-per-year farming system is a specific viticulture management system in Guangxi, China, which allow table grape to be harvested with high production twice a year both in summer and winter. Herein, we used high-throughput sequencing (Illumina Miseq platforms) to investigate the diversity of fungi on the surface of Kyoho grape berries during the ripening process in summer and winter at subtropical Nanning region, Guangxi, China. The results showed that 23 fungal species existed in all samples. Among them, the five most dominant species were Cladosporium ramotenellum, Pseudozyma aphidis, Gyrothrix spp., Gibberella intricans and Acremonium alternatum, with abundance from 61.62% to 91.26%. Analysis using the student’s t-test for Shannon index indicated that components of fungal community varied significantly between the two ripening seasons in a year. The dominant genera of core fungal community were Cladosporium, Gyrothrix, Paramycosphaerella, Acremonium, Penicillium and Tilletiopsis in the summer ripening process and Cladosporium, Pseudozyma, Gibberella, Colletotrichum, Sporobolomyces, Rhodosporidium, Alternaria and Aspergillus in the winter ripening process. Overall, the diversity of fungi on the surface of Kyoho grape berries at Nanning region showed significantly differences between different ripening seasons. Our results contribute to the understanding the characteristics of epiphytic fungal communities on the surface of grape berries in subtropical region.