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Plasma-membrane electrical responses to salt and osmotic gradients contradict radiotracer kinetics, and reveal Na+-transport dynamics in rice (Oryza sativa L.)

Author:
Hamam, Ahmed M., Coskun, Devrim, Britto, Dev T., Plett, Darren, Kronzucker, Herbert J.
Source:
Planta 2019 v.249 no.4 pp. 1037-1051
ISSN:
0032-0935
Subject:
Oryza sativa, chlorides, electrophysiology, enzyme activity, enzyme kinetics, models, phosphates, plasma membrane, potassium, rice, roots, salt stress, seedlings, sodium, sulfates, tracer techniques
Abstract:
MAIN CONCLUSION: A systematic analysis of NaCl-dependent, plasma-membrane depolarization (∆∆Ψ) in rice roots calls into question the current leading model of rapid membrane cycling of Na⁺ under salt stress. To investigate the character and mechanisms of Na⁺ influx into roots, Na⁺-dependent changes in plasma-membrane electrical potentials (∆∆Ψ) were measured in root cells of intact rice (Oryza sativa L., cv. Pokkali) seedlings. As external sodium concentrations ([Na⁺]ₑₓₜ) were increased in a step gradient from 0 to 100 mM, membrane potentials depolarized in a saturable manner, fitting a Michaelis–Menten model and contradicting the linear (non-saturating) models developed from radiotracer studies. Clear differences in saturation patterns were found between plants grown under low- and high-nutrient (LN and HN) conditions, with LN plants showing greater depolarization and higher affinity for Na⁺ (i.e., higher Vₘₐₓ and lower Kₘ) than HN plants. In addition, counterion effects on ∆∆Ψ were pronounced in LN plants (with ∆∆Ψ decreasing in the order: Cl⁻ > SO₄²⁻ > HPO ₄²⁻), but not seen in HN plants. When effects of osmotic strength, Cl⁻ influx, K⁺ efflux, and H⁺-ATPase activity on ∆∆Ψ were accounted for, resultant Kₘ and Vₘₐₓ values suggested that a single, dominant Na⁺-transport mechanism was operating under each nutritional condition, with Kₘ values of 1.2 and 16 mM for LN and HN plants, respectively. Comparing saturating patterns of depolarization to linear patterns of ²⁴Na⁺ radiotracer influx leads to the conclusion that electrophysiological and tracer methods do not report the same phenomena and that the current model of rapid transmembrane sodium cycling may require revision.
Agid:
6332216