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microRNA-144 regulates the NF-κB signaling in miiuy croaker via targeting IL1β

Yan, Xiaolong, Cui, Junxia, Liu, Xuezhu, Xu, Tianjun
Developmental and comparative immunology 2019 v.96 pp. 47-50
Miichthys miiuy, bioinformatics, dose response, enzyme activity, enzyme inhibition, fish, gene overexpression, immune response, interleukin-1beta, luciferase, messenger RNA, microRNA, plasmids, transcription factor NF-kappa B
microRNAs have been demonstrated to be critical regulators of the immune responses. While, the miRNA-mediate the detail regulatory mechanism response is still not clear in fish species. In this research, the regulation of miRNA to the NF-κB signaling through decreasing the target gene mRNAs was discussed in miiuy croaker. We first used the bioinformatics predicted miR-144 has a direct negative regulatory affect on IL1β in miiuy croaker, further the luciferase assays were used to probe the functions of miR-144. The overexpression of miR-144 mimics and pre-miR-144 plasmid all showed the dose-dependent pattern on IL1β. Moreover, the inhibition of luciferase activity was attenuated after co-transfected with miR-144 inhibitors. In addition, we observed that the miR-144 could negative regulate to the nuclear factor kappaB (NF-κB) signaling in miiuy croaker by targeting IL1β. In conclusion, our studies on miR-144 will enlarge knowledge of its functions in regulation of immune response, further provide a new insight to research on the immune regulation mechanism in teleost fish.