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Composition of Mitochondrial Complex I during the Critical Node of Seed Aging in Oryza sativa

Author:
Chen, Baoyin, Yin, Guangkun, Whelan, James, Zhang, Zesen, Xin, Xia, He, Juanjuan, Chen, Xiaoling, Zhang, Jinmei, Zhou, Yuanchang, Lu, Xinxiong
Source:
Journal of plant physiology 2019 v.236 pp. 7-14
ISSN:
0176-1617
Subject:
NAD (coenzyme), Oryza sativa, biogenesis, enzymes, germination, mitochondria, oxidation, oxygen, oxygen consumption, quinones, rice, seedlings, seeds, viability
Abstract:
Previous studies have documented mitochondrial dysfunction during the critical node (CN) of rice (Oryza sativa) seed aging, including a decrease in the capacity of NADH dependent O2 consumption. This raises the hypothesis that changes in the activity of NADH:ubiquinone oxidoreductase (complex I) may play a role in seed aging. The composition and activity of complex I was investigated at the CN of aged rice seeds. Using BN-PAGE and SWATH-MS 52 complex I subunits were identified, nineteen for the first time to be experimentally detected in rice. The subunits of the matrix arm (N and Q modules) were reduced in abundance at the CN, in accordance with a reduction in the capacity to oxidise NADH, reducing substrate oxidation and increase ROS accumulation. In contrast, subunits in the P module increased in abundance that contains many mitochondrial encoded subunits. It is proposed that the changes in complex I abundance subunits may indicate a premature re-activation of mitochondrial biogenesis, as evidenced by the increase in mitochondrial encoded subunits. This premature activation of mitochondrial biogenesis may under-pin the decreased viability of aged seeds, as mitochondrial biogenesis is a crucial event in germination to drive growth before autotrophic growth of the seedling is established.
Agid:
6333658