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Expression of genes controlling steroid metabolism and action in granulosa-lutein cells of women with polycystic ovaries

Lerner, A., Owens, L., Coates, M., Simpson, C., Poole, G., Velupillai, J., Liyanage, M., Christopoulos, G., Lavery, S., Hardy, K., Franks, S.
Molecular and cellular endocrinology 2019
androgens, anovulation, dihydrotestosterone, gene expression, genes, granulosa cells, in vitro fertilization, lutein, oocytes, pathophysiology, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, steroid metabolism, women
Aberrant function of granulosa cells has been implicated in the pathophysiology of PCOS.GL cells were collected during oocyte retrieval for IVF/ICSI. RT-qPCR was used to compare gene expression between 12 control women, 12 with ovulatory PCO and 12 with anovulatory PCOS. To examine which genes are directly regulated by androgens, GL cells from an additional 12 control women were treated in-vitro with 10 nM dihydrotestosterone (DHT).Women with PCOS showed reduced expression of CYP11A1 3-fold (p = 0.005), HSD17B1 1.8-fold (p = 0.02) and increased expression of SULT1E1 7-fold (p = 0.0003). Similar results were seen in ovulatory women with PCO. GL cells treated with 10 nM DHT showed a 4-fold (p = 0.03) increase in expression of SULT1E1 and a 5-fold reduction in SRD5A1 (p = 0.03).These findings support the notion that aberrant regulation of steroid metabolism or action play a part in ovarian dysfunction in PCOS.