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Digital image colorimetric analysis for evaluating lipid oxidation in oils and its emulsion

Singkhonrat, Jirada, Sriprai, Athikhun, Hirunwatthanakasem, Supisara, Angkuratipakorn, Thamonwan, Preechaburana, Pakorn
Food chemistry 2019 v.286 pp. 703-709
cameras, colorimetry, detection limit, digital images, emulsions, farmers, lipid peroxidation, monitoring, oils, peroxide value, portable equipment, quality control, small and medium enterprises, titration, ultraviolet-visible spectroscopy
Optical analytical images were captured by a digital camera, and lipid peroxidation was monitored using colorimetric determination. The Image-J method was shown to have comparable accuracy to the conventional titration (American Oil Chemists’ Society) and UV–Vis spectroscopic methods. Over the PV range 3–14 meq.O2/kg, a good correlation (R2 = 0.9836) was found between the proposed method and UV–Vis method with an SD of ±0.0339 meq.O2/kg. The range and accuracy of detection were determined from the changes in the Image-J intensity values. At equivalent PV, the limit of detection was 0.175 meq.O2/kg and the limit of quantitation was 0.35 meq.O2/kg. The proposed Image-J application can be used to develop fast and portable devices for monitoring peroxide values. These have potential applications in quality control for small-scale farmers and small and medium enterprises.