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Assessment of induced genomic instability in rat primary astrocytes exposed to intermediate frequency magnetic fields

Author:
Herrala, Mikko, Naarala, Jonne, Juutilainen, Jukka
Source:
Environmental research 2019 v.173 pp. 112-116
ISSN:
0013-9351
Subject:
astrocytes, comet assay, flow cytometry, genomics, magnetic fields, menadione, methyl methanesulfonate, mutagens, rats
Abstract:
We investigated whether exposure to intermediate frequency magnetic fields (IF MFs) could induce or enhance genomic instability in primary astrocytes. Rat primary astrocytes were exposed to vertical or horizontal 7.5 kHz, 300 μT MF for 24 h. To study possible combined effects with known genotoxic agents, the cells were exposed for 3 h to menadione or methyl methanesulfonate after the MF treatment. Induced genomic instability was evaluated 36 days after exposures using the Comet assay and flow cytometric scoring of micronuclei. Exposure to 7.5 kHz, 300 μT MF did not induce genomic instability alone or in combination with chemicals in measurements performed several cell generations after exposure.
Agid:
6334725