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Soil-borne wheat mosaic virus infectious clone and manipulation for gene-carrying capacity

Author:
Sridhar Jarugula, Steven R. Charlesworth, Feng Qu, Lucy R. Stewart
Source:
Archives of virology 2016 v.161 no.8 pp. 2291-2297
ISSN:
0304-8608
Subject:
Agrobacterium, Nicotiana benthamiana, RNA, Soil-borne wheat mosaic virus, Triticum aestivum, complementary DNA, gene silencing, genetic vectors, mutagenesis, photobleaching, viruses, wheat
Abstract:
A full-length infectious cDNA clone of soil-borne wheat mosaic virus (SBWMV; genus Furovirus; family Virgaviridae) was developed for agrobacterium delivery. The cloned virus can be agroinfiltrated to Nicotiana benthamiana for subsequent infection of wheat (Triticum aestivum, L.). The utility of the virus as a vector for gene silencing and expression was assessed through sequence insertions in multiple sites of RNA2. Virus-induced photobleaching was observed in N. benthamiana but not in wheat, despite the stability of the inserts. The SBWMV infectious clone can be used for further studies to investigate the biology of SBWMV through mutagenesis.
Agid:
63349
Handle:
10113/63349