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α-lipoic acid regulate growth, antioxidant status and lipid metabolism of Chinese mitten crab Eriocheir sinensis: Optimum supplement level and metabonomics response

Author:
Xu, Chang, Wang, Xiaodan, Han, Fenglu, Qi, Changle, Li, Erchao, Guo, Jianlin, Qin, Jian G., Chen, Liqiao
Source:
Aquaculture 2019 v.506 pp. 94-103
ISSN:
0044-8486
Subject:
Eriocheir sinensis, adverse effects, amino acid metabolism, animal husbandry, antioxidant activity, antioxidants, blood serum, carnitine, crabs, fatty acid metabolism, feed additives, glutathione peroxidase, hepatopancreas, juveniles, lactalbumin, lipoic acid, messenger RNA, metabolites, metabolomics, superoxide dismutase, weight gain
Abstract:
The α-lipoic acid (α-LA) is a novel feed additive to improve growth, antioxidant capacity and lipid metabolism in animal husbandry. In this study, isonitrogenous and isoenergetic diets were formulated at seven levels of α-LA (0, 300, 600, 1200, 2400, 4800, or 9600 mg/kg) and fed to the Chinese mitten crab Eriocheir sinensis juveniles for eight weeks. According to weight gain and specific grwoth rate, the optimal level of α-LA supplement in the diet is estimated at 1339–1574 mg/kg for E. sinensis. The α-LA supplement significantly increased the activities of SOD, GSH-Px and T-AOC, and the content of GSH in the hepatopancreas except for SOD in the crabs fed 9600 mg/kg α-LA. Excess dietary α-LA supplement increased the content of MDA in the hepatopancreas. The E. sinensis fed 2400 mg/kg α-LA in the diet showed the highest mRNA expressions of es_TGL1, es_TGL2, es_IL and es_DGL and had higher lipid catabolism when taking 300 mg/kg α-LA than other diets, which coincides with higher mRNA expressions of es_IL, es_DGL and CPT-1 in the hepatopancreas. The crab fed 2400 mg/kg α-LA significantly modified 45 metabolites in serum compared with the control. The supplmentation of α-LA significantly influenced fatty acid metabolism, amino acid metabolism, carnitine metabolism and accumulation of plant antioxidants. This study indicates that α-LA is a promising feed additive to regulate growth, antioxidant status and lipid metabolism in E. sinensis, but its inclusion in the diet should be <2400 mg/kg α-LA to maximize the benefitial effect and minimize side effects.
Agid:
6335468