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Molecular taxonomic evaluation of 40 Anabaena and 40 Nostoc isolates from the third largest European soil microalgal culture collection

Author:
Makra, N., Gell, G., Juhász, A., Soós, V., Kiss, T., Molnár, Z., Ördög, V., Vörös, L., Balázs, E.
Source:
South African journal of botany 2019
ISSN:
0254-6299
Subject:
Anabaena, Nostoc, algae culture, algorithms, monophyly, nucleotide sequences, paraphyly, ribosomal RNA, soil, taxonomy
Abstract:
The taxonomy of genera Anabaena and Nostoc is very controversial. They are typically paraphyletic within phylogenetic trees and show similar morphological characters. Our study aimed to determine the taxonomic relationships among Anabaena and Nostoc isolates of the Mosonmagyaróvár Algal Culture Collection (MACC) using 16S rRNA and rbcLX gene sequences. We concluded on the basis of the number of unsuccessful amplifications that Anabaena isolates are more non-axenic than Nostoc isolates. In agreement with previous studies we noticed that the applied phylogenetic algorithms gave congruent results in phylogenetic analyses. However, the genus Nostoc was found clearly not monophyletic in our study and this finding differed from many of the previous studies. Result of the BLAST algorithm contradicted with the previous morphology based classification of some MACC cyanobacteria strains, therefore multiple taxonomic approaches are required for the reliable identification of cyanobacterial species. Some isolates seemed to be identical based on the alignment of 16S rRNA or rbcLX sequences.
Agid:
6336496