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The enhancement of bioactive potential in Vitis vinifera leaves by application of microspheres loaded with biological and chemical agents

Vinceković, Marko, Maslov Bandić, Luna, Jurić, Slaven, Jalšenjak, Nenad, Čaić, Ana, Živičnjak, Irena, Đermić, Edyta, Karoglan, Marko, Osrečak, Mirela, Topolovec-Pintarić, Snježana
Journal of plant nutrition 2019 v.42 no.6 pp. 543-558
Trichoderma viride, Vitis vinifera, alginates, antioxidant activity, beta-carotene, bioactive compounds, calcium, cations, chlorophyll, flowering, functional foods, grapes, leaves, magnesium, microparticles, polyphenols, small fruits, vines
An overview of a research on Vitis vinifera plants treated with a chemical (calcium and magnesium ions) and biological (Trichoderma viride) agents simultaneously loaded in alginate microspheres was presented. Microspheres were applied at two growth stages: before flowering and berries pea-size. Physicochemical characteristics of leaves after the two growth stages and grapes were measured in terms of bioactive components content and antioxidant activity. After the treatments, vine leaves reached a significant increase in almost all measured parameters (polyphenols, antioxidant capacity, β-carotene, and chlorophyll) compared to the control. The highest total chlorophyll content was found after the treatment with microspheres containing Mg²⁺/Ca²⁺ cations, and T. viride. The treatments enhanced Vitis vinifera leaves in terms of bioactive potential and can be further used as a functional food. As compared to the control, somewhat elevated values of total polyphenols and antioxidant activity were found on the grape samples.