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Silicon-induced antioxidant defense and methylglyoxal detoxification works coordinately in alleviating nickel toxicity in Oryza sativa L.

Author:
Hasanuzzaman, Mirza, Alam, Md. Mahabub, Nahar, Kamrun, Mohsin, Sayed Mohammad, Bhuyan, M. H. M. Borhannuddin, Parvin, Khursheda, Hawrylak-Nowak, Barbara, Fujita, Masayuki
Source:
Ecotoxicology 2019 v.28 no.3 pp. 261-276
ISSN:
0963-9292
Subject:
Oryza sativa, antioxidant activity, biomass production, chlorophyll, chlorosis, cytotoxicity, enzymes, growth retardation, hydroponics, leaves, lipids, nickel, oxidative stress, peroxidation, plant growth, proline, reactive oxygen species, rice, seedlings, silicon
Abstract:
Nickel (Ni), an essential nutrient of plant but very toxic to plant at supra-optimal concentration that causes inhibition of seed germination emergence and growth of plants as a consequence of physiological disorders. Hence, the present study investigates the possible mechanisms of Ni tolerance in rice seedlings by exogenous application of silicon (Si). Thirteen-day-old hydroponically grown rice (Oryza sativa L. cv. BRRI dhan54) were treated with Ni (NiSO₄.7H₂O, 0.25 and 0.5 mM) sole or in combination with 0.50 mM Na₂SiO₃ for a period of 3 days to investigate the effect of Si supply for revoking the Ni stress. Nickel toxicity gave rise to reactive oxygen species (ROS) and cytotoxic methylglyoxal (MG), accordingly, initiated oxidative stress in rice leaves, and accelerated peroxidation of lipids and consequent damage to membranes. Reduced growth, biomass accumulation, chlorophyll (chl) content, and water balance under Ni-stress were also found. However, free proline (Pro) content increased in Ni-exposed plants. In contrast, the Ni-stressed seedlings fed with supplemental Si reclaimed the seedlings from chlorosis, water retrenchment, growth inhibition, and oxidative stress. Silicon up-regulated most of the antioxidant defense components as well as glyoxalase systems, which helped to improve ROS scavenging and MG detoxification. Hence, these results suggest that the exogenous Si application can improve rice seedlings’ tolerance to Ni-toxicity.
Agid:
6337415