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Designing of an epitope-based peptide vaccine against walking pneumonia: an immunoinformatics approach

Unni, P. Ambili, Ali, A. M. Mohamed Thoufic, Rout, Madhusmita, Thabitha, A., Vino, S., Lulu, S. Sajitha
Molecular biology reports 2019 v.46 no.1 pp. 511-527
B-lymphocytes, Mycoplasma pneumoniae, T-lymphocytes, binding capacity, epitopes, immune response, immunoinformatics, pathogens, peptides, pneumonia, proteins, screening, therapeutics, vaccine development, vaccines
Mycoplasma pneumoniae is a substantial respiratory pathogen that develops not only pneumonia but also other respiratory diseases, which mimic viral respiratory syndromes. Nevertheless, vaccine development for this pathogen delays behind as immunity correlated with protection is now predominantly unknown. In the present study, an immunoinformatics pipeline is utilized for epitope-based peptide vaccine design, which can trigger a critical immune response against M. pneumoniae. A total of 105 T-cell epitopes from 12 membrane associated proteins and 7 T-cell epitopes from 5 cytadherence proteins of M. pneumoniae were obtained and validated. Thus, 18 peptides with 9-mer core sequence were identified as best T-cell epitopes by considering the number of residues with > 75% in favored region. Further, the crucial screening studies predicted three peptides with good binding affinity towards HLA molecules as best T-cell and B-cell epitopes. Based on this result, visualization, and dynamic simulation for the three epitopes (WIHGLILLF, VILLFLLLF, and LLAWMLVLF) were assessed. The predicted epitopes needs to be further validated for their adept use as vaccine. Collectively, the study opens up a new horizon with extensive therapeutic application against M. pneumoniae and its associated diseases.