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Aloin reduces inflammatory gene iNOS via inhibition activity and p-STAT-1 and NF-κB

Lee, Wonhwa, Yang, Sumin, Lee, Changhun, Park, Eui Kyun, Kim, Kyung-Min, Ku, Sae-Kwang, Bae, Jong-Sup
Food and chemical toxicology 2019 v.126 pp. 67-71
Aloe, anthraquinones, antioxidant activity, cytosol, enzyme activity, genes, glycosides, heme oxygenase (biliverdin-producing), human umbilical vein endothelial cells, inducible nitric oxide synthase, interleukin-1beta, lipopolysaccharides, lungs, mice, nitric oxide, phosphorylation, prostaglandin synthase, protein synthesis, toxicology, transcription factor NF-kappa B, tumor necrosis factor-alpha
Aloin is the major anthraquinone glycoside obtained from the Aloe species and exhibits anti-inflammatory and anti-oxidative activities. Here, we aimed to determine the effects of aloin on heme oxygenase-1 (HO-1) induction and on the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX) 2 in lipopolysaccharide (LPS)-activated human umbilical vein endothelial cells (HUVECs). To the end, aloin was tested whether aloin reduces iNOS protein expression and inflammatory markers (interleukin (IL)-1β and tumor necrosis factor (TNF)-α) in LPS-treated mice lung tissue. The results indicated that aloin affected HO-1 induction and reduced LPS-activated NF-κB-luciferase activity showed to preferential inhibition of iNOS/NO and COX-2/PGE2 that was partly related to inhibition of STAT-1 phosphorylation. In particular, aloin induced translocation of Nrf2 from cytosol into the nucleus by an increased Nrf2-ARE binding activity, and reduced IL-1β production in LPS-activated HUVECs. The reduced expression of iNOS/NO by aloin was reversed by siHO-1RNA-transfection. In LPS-treated mice, aloin significantly reduced iNOS protein in lung tissues, and TNF-α levels in the BALF. We concluded that aloin may be beneficial for treatment of lung injury.