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An eco-designed paper-based algal biosensor for nanoformulated herbicide optical detection

Author:
Scognamiglio, Viviana, Antonacci, Amina, Arduini, Fabiana, Moscone, Danila, Campos, Estefania V.R., Fraceto, Leonardo F., Palleschi, Giuseppe
Source:
Journal of hazardous materials 2019 v.373 pp. 483-492
ISSN:
0304-3894
Subject:
Chlamydomonas reinhardtii, agar, arsenic, biosensors, copper, copper nanoparticles, cost effectiveness, dose response, fluorescence, glass, herbicidal properties, herbicides, photosynthetic algae, storage quality, tap water
Abstract:
In this study we reported the development of a paper-based algal biosensor for the optical detection of nanoencapsulated-atrazine, a forefront nanoformulated herbicide with a high effective post-emergence herbicidal activity. In particular, the unicellular green photosynthetic algae Chlamydomonas reinhardtii was immobilised on a paper substrate soaked with an agar thin film and placed in a glass optical measurement cell, obtaining a totally environmental-friendly device. Nanoencapsulated-atrazine was detected by following the variable fluorescence (1-VJ) parameter, which decreased inversely proportional to the herbicide concentrations, in a range between 0.5 and 200 nM, indicating a linear relationship in the measured dose-response curves and a detection limit of 4 pM. Interference studies resulted in a very slight interference in presence of 2 ppm copper and 10 ppb arsenic at safety limits, as well as a slight matrix effect and a satisfactory recovery value of 96 ± 5% for 75 nM nanoencapsulated-atrazine in tap water. Stability studies were also performed obtaining a good storage stability up to 3 weeks. Results demonstrated the suitability of the proposed paper-based optical biosensor as a valid support in smart agriculture for on site, environmental friendly, cost effective and sensitive nanoencapsulated-atrazine analysis.
Agid:
6338531