Main content area

An eco-designed paper-based algal biosensor for nanoformulated herbicide optical detection

Scognamiglio, Viviana, Antonacci, Amina, Arduini, Fabiana, Moscone, Danila, Campos, Estefania V.R., Fraceto, Leonardo F., Palleschi, Giuseppe
Journal of hazardous materials 2019 v.373 pp. 483-492
Chlamydomonas reinhardtii, agar, arsenic, biosensors, copper, copper nanoparticles, cost effectiveness, dose response, fluorescence, glass, herbicidal properties, herbicides, photosynthetic algae, storage quality, tap water
In this study we reported the development of a paper-based algal biosensor for the optical detection of nanoencapsulated-atrazine, a forefront nanoformulated herbicide with a high effective post-emergence herbicidal activity. In particular, the unicellular green photosynthetic algae Chlamydomonas reinhardtii was immobilised on a paper substrate soaked with an agar thin film and placed in a glass optical measurement cell, obtaining a totally environmental-friendly device. Nanoencapsulated-atrazine was detected by following the variable fluorescence (1-VJ) parameter, which decreased inversely proportional to the herbicide concentrations, in a range between 0.5 and 200 nM, indicating a linear relationship in the measured dose-response curves and a detection limit of 4 pM. Interference studies resulted in a very slight interference in presence of 2 ppm copper and 10 ppb arsenic at safety limits, as well as a slight matrix effect and a satisfactory recovery value of 96 ± 5% for 75 nM nanoencapsulated-atrazine in tap water. Stability studies were also performed obtaining a good storage stability up to 3 weeks. Results demonstrated the suitability of the proposed paper-based optical biosensor as a valid support in smart agriculture for on site, environmental friendly, cost effective and sensitive nanoencapsulated-atrazine analysis.