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Effects of Siwu decoction on chondrocyte proliferation of growth plate in adolescent rats
- Lee, Donghun, Lee, Sun Haeng, Lee, Minwoo, Lee, Sung Hyun, Shin, Yu Jeong, Lee, Jin Yong, Kim, Hocheol, Kim, Young-Sik, Song, Jungbin
- Journal of ethnopharmacology 2019 v.236 pp. 108-113
- adolescents, blood, bone formation, bone morphogenetic proteins, children, chondrocytes, females, fluorescence, growth plate, growth retardation, immunohistochemistry, insulin-like growth factor I, rats, somatotropin, tetracycline, tibia, traditional medicine
- According to traditional Korean medicine theory in which children's growth retardation is attributed to blood deficiency, Siwu decoction (SWD), a representative treatment for blood deficiency, was chosen as a sample.Aim of the study: To evaluate the effects of SWD on chondrocyte proliferation of growth plate in adolescent female rats.Female adolescent rats were allocated to one of the following four groups; SWD 100 and 300 mg/kg, recombinant human growth hormone, and vehicle for 4 days. Tetracycline was intraperitoneally injected at 48 h before sacrifice to obtain a band exhibiting fluorescence by binding newly formed bone. Bromodeoxyuridine was injected at day 2–4 to mark proliferating chondrocytes. To evaluate possible mechanisms of SWD, expressions of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-2 (BMP-2) in the growth plate were examined by immunohistochemistry.Treatment with SWD significantly increased the number of BrdU-positive chondrocytes and the new bone formation in the proximal growth plate of tibia compared to the vehicle treated control group. SWD also increased the expression of IGF-1 and BMP-2 in the proliferative and hypertrophic zones of the growth plate.SWD 300 mg/kg stimulates chondrocyte proliferation and new bone formation in the growth plate. Immunohistochemical studies indicate that the effects of SWD may be due to upregulation of local IGF-1 and BMP-2 expression in the growth plate, which may be considered as a GH-dependent paracrine-autocrine pathway.