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Treatment and control of mycoplasma contamination in Plasmodium falciparum culture

Author:
Singh, Shubhra, Puri, S. K., Srivastava, Kumkum
Source:
Parasitology research 2008 v.104 no.1 pp. 181-184
ISSN:
0932-0113
Subject:
Mycoplasma, Plasmodium falciparum, death, malaria, parasites, tetracycline
Abstract:
A comparative efficacy of four antibiotics, plasmocin (macrolid), Biomyc-1, -2, (tetracycline), and Biomyc-3, and Mycoplasma Removing Agent (quinolone derivatives) was determined for elimination of mycoplasma from Plasmodium falciparum culture. Presence of mycoplasma was detected using enzyme-PCR-based mycoplasma detection kit and survival of malaria parasite was determined in Giemsa’s stained smear made from treated and untreated cultures. It was observed that a combination of Biomyc-1 and -2 killed malaria parasites within 24 h, whereas plasmocin and Biomyc-3 caused slow death of malaria parasite stretched over a period of 6 days. The only compound which did not kill malaria parasite and eradicated mycoplasma from P. falciparum culture was observed to be MRA.
Agid:
634016