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Constructing high effective nano-Mn3(PO4)2-chitosan in situ electrochemical detection interface for superoxide anions released from living cell
- Wang, Ying, Wang, Deng, Sun, Li-Hong, Xue, Peng, Wang, Min-Qiang, Lu, Zhisong, Wang, Feng, Xia, Qingyou, Xu, Mao-Wen, Bao, Shu-Juan
- Biosensors & bioelectronics 2019 v.133 pp. 133-140
- biocompatibility, biosensors, breast neoplasms, cell culture, cell lines, chitosan, electrochemistry, electrodes, electron paramagnetic resonance spectroscopy, mice, monitoring, nanowires, neoplasm cells, superoxide anion, toxicity
- Monitoring superoxide anions in living cells have attracted much academic and biomedical interest due to their important role in metabolic processes. Herein, we confined ultra-small nano-Mn3(PO4)2 in chitosan and designed a unique puffy woven sphere consisted by nanowires. Further constructed an effective in situ detection chip using the as-synthesized nano-Mn3(PO4)2-chitosan for electrochemical sensing of superoxide anions from murine breast tumor cells (4T1). The excellent biocompatibility of chitosan and large size of the Mn3(PO4)2-chitosan spheres greatly reduced the damage and toxicity of the detection interface to the living cells, while the ultra-small nano-Mn3(PO4)2 in chitosan could effectively catalyze the superoxide anions released from cells. The nano-Mn3(PO4)2-chitosan-based sensor exhibited high sensitivity (1.6 μA μM−1), low detection limit (9.4 nM at S/N = 3) and good selectivity for O2•−. After cell culture on the surface of nano-Mn3(PO4)2-chitosan based electrode. As a miniature analytical and sensing platform, results further suggest that the prepared chip offers a more sensitive detective superoxide anions (O2•−) released from 4T1 cell lines than traditional electron paramagnetic resonance (EPR) analysis.