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Influence of different cleaning and sanitisation procedures on the removal of adhered Bacillus cereus spores

Ribeiro, Maria Cecília E., da Silva Fernandes, Meg, Kuaye, Arnaldo Yoshiteru, Gigante, Mirna L.
International dairy journal 2019 v.94 pp. 22-28
Bacillus cereus, UHT milk, adhesion, analysis of variance, cleaning, dairy industry, detection limit, equipment, milk, peracetic acid, sanitation, sodium hypochlorite, spores, water quality
The effectiveness of nine different sanitation procedures for the removal and control of adhered Bacillus cereus spores isolated from the dairy industry was evaluated. Four sets of experiments were produced by varying the conditioning matrix (water and UHT whole milk) and the inoculation medium of the pool of B. cereus spores (water and UHT whole milk), resulting in Ewater/water, Ewater/milk, Emilk/water, and Emilk/milk. The experiments were repeated three times and evaluated by ANOVA and Tukey's test. Sanitisation with sodium hypochlorite was more effective than peracetic acid for removal of adhesion, especially when associated with a cleaning procedure, with counts below the limit of detection (<0.7 log cfu cm−2). The experiments formed when the pool of spores was inoculated on water (Ewater/water and Emilk/water) presented greater resistance against the sanitation procedures studied. These results highlight the importance of the water quality used in the industries for sanitation of equipment.