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Effect of the isoelectric point of pH-responsive lignin-based amphoteric surfactant on the enzymatic hydrolysis of lignocellulose

Zhan, Xuejuan, Cai, Cheng, Pang, Yuxia, Qin, Feiyang, Lou, Hongming, Huang, Jinhao, Qiu, Xueqing
Bioresource technology 2019 v.283 pp. 112-119
Eucalyptus, adsorption, endo-1,4-beta-glucanase, enzymatic hydrolysis, enzyme activity, isoelectric point, lignin, lignocellulose, quaternary ammonium compounds, saccharification, surfactants
The isoelectric point (pI) of lignin-based surfactant is an important factor in the enhancement on the enzymatic hydrolysis of lignocellulose. In this work, lignin carboxylate (LC) and quaternary ammonium lignin carboxylates (LCQ-x, x%: the mass ratio of quaternizing agent to enzymatic hydrolysis lignin) with different isoelectric points were synthesized. LC or LCQ-x with pI significantly lower or higher than 4.8 reduced the non-productive adsorption of cellulase on lignin, but for the significant inhibitory effect on cellulase activity, their enhancements on the enzymatic hydrolysis of lignocellulose were not remarkable. However, LCQ-x with pI around 4.8 preserved the cellulase activity, and significantly reduced the non-productive adsorption of cellulase, therefore remarkably enhanced the enzymatic hydrolysis. 2 g/L LC, LCQ-40 (pI = 5.0) and LCQ-100 (pI = 9.2) increased the enzymatic digestibility of pretreated eucalyptus from 35.2% to 53.4%, 95.3% and 60.4% respectively. In addition, for the excellent pH-response performance, LCQ could be efficiently recovered after enzymatic saccharification.