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Improvement of continuous hydrogen production using individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors

Montoya-Rosales, José de Jesús, Olmos-Hernández, Diana Karime, Palomo-Briones, Rodolfo, Montiel-Corona, Virginia, Mari, Angelo Gabriel, Razo-Flores, Elías
Bioresource technology 2019 v.283 pp. 251-260
bagasse, biofilm, biofuels, energy, hydrogen, hydrogen production, hydrolysates, hydrolysis
Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR). The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively. Meanwhile, VHPR of 5.76 and 2.0 L H2/L-d were obtained in the TBR configuration using BH and IH, respectively. Differences on VHPR between reactors could be explained by substrate availability, which is intrinsic to the growth mode of each reactor configuration; while differences of VHPR between hydrolysates were possibly related to the composition of enzymatic hydrolysates. Furthermore, homoacetogenesis was strongly influenced by H2 and substrate transfer conditions. Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.