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A review on methodologies for extraction, identification and quantification of allergenic proteins in prawns

Author:
Faisal, M., Vasiljevic, T., Donkor, O.N.
Source:
Food research international 2019 v.121 pp. 307-318
ISSN:
0963-9969
Subject:
allergenicity, allergens, circular dichroism spectroscopy, enzyme-linked immunosorbent assay, high performance liquid chromatography, hypersensitivity, immunoblotting, polyacrylamide gel electrophoresis, shrimp
Abstract:
Prawn allergy is one of the most common food-borne allergies and current prevention is by avoidance. This review paper summarised different methodologies for the extraction, identification and quantification of prawn protein allergens, reported in various research studies. Following extraction, allergenic components have been analysed using well-established methodologies, such as SDS-PAGE, Immunoblotting, ELISA, CD Spectroscopy, HPLC, DBPCFC, SPT etc. Moreover, the preference towards Aptamer-based technique for allergenicity analysis has also been highlighted in this review paper. The summary of these methodologies will provide a reference platform for present and future research directions.
Agid:
6341129