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Analysis of pYC2, a cryptic plasmid in Lactobacillus sakei BM5 isolated from kimchi
- Yang, Eun Ju, Chang, Hae Choon
- Biotechnology letters 2009 v.31 no.1 pp. 123-130
- Lactobacillus sakei, Southern blotting, kimchi, open reading frames, plasmids, proteins, sequence analysis
- Analysis of the structural properties of pYC2, a cryptic plasmid from Lactobacillus sakei BM5 isolated from kimchi, determined its length as 1,970 bp with a G + C content of 34%. The double-strand origin (dso) and single-strand origin (sso) of rolling-circle replicating plasmids were found in the nucleotide sequence of the pYC2 plasmid. Sequence analysis of pYC2 revealed that ORF 1 and ORF 2 showed high homology with the Cop and Rep proteins encoded by the pMV158 family of plasmids replicating via the rolling-circle mechanism. pYC2 also replicates by this mechanism, as confirmed by Southern hybridization analysis.