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Expression of stilbene synthase gene in transgenic tomato using salicylic acid-inducible Cre/loxP recombination system with self-excision of selectable marker
- Ma, B. G., Duan, X. Y., Niu, J. X., Ma, C., Hao, Q. N., Zhang, L. X., Zhang, H. P.
- Biotechnology letters 2009 v.31 no.1 pp. 163-169
- Vitis vinifera, genes, genetic markers, kanamycin kinase, salicylic acid, tomatoes, transgenic plants
- A plant transformation vector, pCLKSCLA25 (EU327498), was developed to contain eight cloning sites and the inducible self-excision system which provided an effective approach to eliminate the selectable marker gene(s) from transgenic plants. Upon induction by salicylic acid, the cre gene produced a recombinase that eliminated sequences encoding the selectable marker neomycin phosphotransferase and cre itself. The excision efficiency was 41% in transgenic tomato regenarants. The stilbene synthase gene (vst1) from Vitis vinifera L. was cloned into pCLKSCLA25. The expression of vst1 gene contributed to the accumulation of trans-reveratrol from 3.4 to 8.7 μg/g fresh wt in different marker-free transgenic tomato lines.