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Adaptation of a Mycobacterium strain to phenanthrene degradation in a biphasic culture system: influence on interfacial area and droplet size
- Abdelhay, Arwa, Magnin, Jean-Pierre, Gondrexon, Nicolas, Baup, Stéphane, Willison, John
- Biotechnology letters 2009 v.31 no.1 pp. 57-63
- Mycobacterium, biofilm, carbon, droplet size, emulsions, inoculum, phenanthrene, salts, silicone
- A phenanthrene-degrading Mycobacterium sp. strain 6PY1 was grown in an aqueous/organic biphasic culture system with phenanthrene as sole carbon source. Its capacity of degradation was studied during sequential inoculum enrichments, reaching complete phenanthrene degradation at a maximim rate of 7 mg l⁻¹ h⁻¹. Water–oil emulsions and biofilm formation were observed in biphasic cultures after four successive enrichments. The factors influencing interfacial area in the emulsions were: the initial phenanthrene concentration, the initial inoculum size, and the silicone oil volume fraction. The results showed that the interfacial area was mainly dependent on the silicone oil/mineral salts medium ratio and the inoculum size.